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Reversed phase HPLC of different membrane‐bound variant surface glycoprotein preparations from Trypanosoma brucei brucei
Author(s) -
Tetaert Daniel,
Hublart Marylègne,
Gomès Véronique,
Boersma Arnold,
Degand Pierre
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030203
Subject(s) - chromatography , trypanosoma brucei , chemistry , high performance liquid chromatography , trifluoroacetic acid , elution , glycoprotein , extraction (chemistry) , membrane , reversed phase chromatography , biochemistry , gene
The chromatographic behaviour of the membrane‐attached variant surface glycoprotein (mfVSG) of bloodstream forms of Trypanosoma brucei brucei AnTat 1.1 A preparations were studied by reversed phase high performance liquid chromatography (RP‐HPLC). Among the different preparation procedures used, only the trifluoroacetic acid extraction gave a mfVSG preparation which was eluted from the RP‐HPLC column. As well as for the soluble variant surface glycoprotein (sVSG) it was found that different mfVSG forms were eluted at different organic solvent concentrations from the RP‐HPLC column. In addition, in preliminary studies, we have attempted to characterize the factors responsible for the trypanosome surface coat assembly. Using chromatographic techniques (RP‐HPLC and thin layer chromatography), the results suggest a close relationship between the content of lipid‐containing species and the heterogeneity of mfVSG preparation.