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Simultaneous measurement of ethosuximide and phenobarbital in brain tissue, serum and urine by HPLC
Author(s) -
GalanValiente Josefa,
SotoOtero Ramon,
SierraMarcuño German
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030202
Subject(s) - chemistry , chromatography , dichloromethane , urine , phenobarbital , glucuronide , extraction (chemistry) , high performance liquid chromatography , solvent , biochemistry , pharmacology , medicine
A simple rapid method for simultaneous ethosuximide and phenobarbital assay in brain tissue, serum and urine has been developed. Extraction of samples from brain tissue and serum were performed with dichloromethane at low pH in the presence of an excess of ammonium sulfate. Glucuronide conjugates in urine samples were hydrolyzed by enzymatic cleavage with β‐glucuronidase and then extracted with dichloromethane. The extracts were analyzed using a Spherisorb 5 ODS column and a mixture of acetonitrile, methanol and phosphate buffer (21: 24: 55, v/v) as eluent. No interference was encountered and the method is both precise and reproducible.