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Solid phase extraction and HPLC determination of spiramycin in plasma and vitreous concentrations
Author(s) -
Carlhant D.,
Le Bot M. A.,
Guedes Y.,
Riche C.,
Mimouri F.,
Colin J.,
Berthou F.
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030102
Subject(s) - spiramycin , chemistry , chromatography , high performance liquid chromatography , extraction (chemistry) , cartridge , pharmacokinetics , solid phase extraction , detection limit , coefficient of variation , antibiotics , erythromycin , pharmacology , biochemistry , mechanical engineering , engineering , medicine
A method for extracting spiramycin by an octadecylsilica cartridge is described for plasma or vitreous samples. The macrolide antibiotic is then measured by reversed‐phase HPLC with UV detection. The limit of detection is estimated to be 50 ng/mL. The coefficient of variation for the procedure is 6.1% and 5.2% for the range of concentrations 0.2 μg/mL and 10 μg/mL respectively. By this method, pharmokinetic profiles were performed for five adult patients. Spiramycin could be accurately measured in the vitreous humour, allowing the determination of antibiotic at its site of action.