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MicroHLPC determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae
Author(s) -
He LiYi,
Li BaoMing
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020609
Subject(s) - amygdalin , chemistry , semen , chromatography , biology , medicine , genetics , alternative medicine , pathology
The application of micro HPLC to the determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae is described. Amygdalin is separated at ambient temperature on a reversed phase column of U‐Finepak SIL C 18 (150×0.5 mm) with methanol + water (25: 75 v/v) as the mobile phase at a flow rate of 10 μL/min. The results are calculated by the internal standard method. The linear range is 1–7 μg. The CV and recovery of pure amygdalin are 1.47% ( n = 10) and 98.13%, respectively. The results of analysis are lower than those obtained by TLC, but microHPLC is much simpler, faster, and more sensitive and reproducible than TLC.