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The determination of selenomethionine in selenium yeast by cyanogen bromide gas chromatography
Author(s) -
Ouyang Zheng,
Wu Jiang
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020607
Subject(s) - chemistry , cyanogen bromide , selenium , chromatography , gas chromatography , ion chromatography , cyanogen , yeast , hydrolysis , biochemistry , organic chemistry , peptide sequence , gene
A new method for the determination of bonded selenomethionine in selenium yeast by gas chromatography is established. Bonded selenomethionine is rapidly and precisely determined by measuring CH 3 SeCN released during the reaction of selenoprotein with CNBr (CNBr‐GC method). Conditions for the reaction and chromatography are described. The results of CNBr‐GC are compared with those obtained by acid hydrolysis/ion exchange chromatography. This new method has the advantage of being accurate, sensitive and selective.