Premium
Determination of 4‐methylpyrazole in plasma using solid phase extraction and HPLC
Author(s) -
Diczfalusy Ulf,
Eklöf Roland
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020511
Subject(s) - chemistry , chromatography , extraction (chemistry) , elution , phosphate buffered saline , high performance liquid chromatography , solid phase extraction , plasma , linear range , phosphate , gradient elution , detection limit , biochemistry , physics , quantum mechanics
A rapid method for the determination of 4‐methylpyrazole in plasma is described. Internal standard (3‐methylpyrazole) is added to the plasma which is subsequently applied to a BondElut SCX column. After washing the column the 3‐ and 4‐methylpyrazoles are eluted with a phosphate buffer and analyzed by isocratic reversed‐phase high performance liquid chromatography with UV detection. The concentration of 4‐methylpyrazole is calculated from the 4‐methylpyrazole/3‐methylpyrazole peak high ratio. The method is linear between 2.5 and 100 μmol/L with a within‐day precision (CV) of 2.2% ( n = 10) and a day‐to‐day precision of 2.8% ( n = 30). The sensitivity is sufficient for analysis of plasma levels in the low micromolar range.