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The determination of cysteamine in physiological fluids by HPLC with electrochemical detection
Author(s) -
Kelly Michael J.,
Perrett David,
Rudge Susan R.
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020509
Subject(s) - chemistry , cysteamine , chromatography , urine , dithiothreitol , detection limit , selectivity , thiol , electrode , high performance liquid chromatography , electrochemistry , quantitative analysis (chemistry) , biochemistry , catalysis , enzyme
Cysteamine, an amino thiol, was separated by rapid isocratic cation exchange chromatography and detected by electrochemical oxidation at a platinum electrode maintained at +0.45 V relative to an Ag/AgCl reference electrode. Eluent pH and electrode working potentials were optimized and the effects of alternative buffers and organic modifiers have been examined. On column sensitivity for cysteamine was 1.5 pmol at a signal‐to‐noise ratio of 5. Although the specificity was good, plasma samples required maximal sensitivity whereas urine samples required greater selectivity, which was achieved by use of lower working potentials. Cysteamine concentrations were determined in serial samples of plasma and urine from volunteers who had received a single oral dose of 200 mg of the drug. Cysteamine was rapidly oxidized in vivo , and detection required prior reduction with dithiothreitol before analysis.