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Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma
Author(s) -
Mazzini M. N.,
Di Giacomo M. A.,
Cerezo A. S.
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020405
Subject(s) - chemistry , chromatography , glycopeptide , human plasma , hydrophilic interaction chromatography , fractionation , glycosidic bond , high performance liquid chromatography , organic chemistry , biochemistry , enzyme , antibiotics
Hydrophobic interaction chromatography was employed for the fractionation of the glycopeptide mixture obtained after autoproteolysis and delipidization treatment of normal human seminal plasma samples. This sequence of procedures led to the elimination of highly hydrophobic components and to the concentration of the carbohydrate‐rich fractions. A partial separation between the O ‐ and N ‐glycosidic‐linked oligopeptides was also achieved after the chromatographic step.