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A rapid and sensitive method for measurement of leukotrienes based on HPLC
Author(s) -
Odlander Björn,
Claesson HansErik
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020403
Subject(s) - chemistry , chromatography , elution , high performance liquid chromatography , methanol , leukotriene d4 , leukotriene , gradient elution , receptor , biochemistry , antagonist , medicine , organic chemistry , asthma
A rapid and sensitive method for simultaneous detection of leukotriene (LT) B 4 , LTC 4 , LTD 4 , LTE 4 , 5S,12S‐dihydroxy‐eicosatetraenoic acid, 6‐trans LTB 4 , 12‐epi‐6‐ trans LTB 4 and the ω‐metabolites of LTB 4 has been developed. Leukocyte incubations were terminated by the addition of one volume of methanol, and the mixture was centrifuged. Analysis was then directly performed by reverse phase high performance liquid chromatography on a column packed with 3 μm Nucleosil C 18 guarded by a precolumn. Quantitation of eluted compounds was carried out by UV‐monitoring at 280 nm and integration of the elution profile. The sensitivity of the method was approximately 1 ng for both LTB 4 and LTC 4 . Accuracy was 101.6% (SD ± 9.8%) for LTB 4 and 99.9% (SD ± 8.7%) for LTC 4 in the interval 5–160 ng.