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Rapid and simple HPLC determination of 5,5‐dimethyl‐2,4‐oxazolidynedione in rabbit serum
Author(s) -
Gaździk Wiesław R.,
Filipek Marian,
Kmiotek Witold
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020310
Subject(s) - chemistry , chromatography , barbital , high performance liquid chromatography , rabbit (cipher) , detection limit , mathematics , statistics
Abstract A new method for the determination of 5,5‐dimethyl‐2,4‐oxazolidynedione (DMO) in rabbit serum by reversed phase‐HPLC with UV detection is described. The determination of DMO is performed without derivatisation. The internal standard is 5,5‐diethylbarbituric acid (barbital). The method is rapid and simple with sensitivity limit of 20 ng/mL, high recovery (above 92%) and is suitable for pharmacokinetic studies.