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Separation and quantitation of plasma free fatty acids as phenacyl esters by HPLC
Author(s) -
Uji Yoshinori,
Noma Akio,
Shiraki Masataka,
Maeda Masako,
Tsuji Akio,
Okabc Hiroaki
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020306
Subject(s) - chemistry , chromatography , phenacyl , high performance liquid chromatography , fatty acid , detection limit , gas chromatography , organic chemistry
We have developed a rapid method for the separation of plasma free fatty acids as their phenacyl esters by high‐performance liquid chromatography (HPLC) using a reversed‐phase (C 18 ) column. The derivatives of series of both saturated and unsaturated fatty acids (C 12:0 C 22:6 ) are simultaneously separated within 45 min and detected with ultraviolet at 241 nm. The limit of detection of fatty acids was approximately 0.5 nmol in 20 μL injected volume of extracts, and the coefficient of variation of the present method did not exceed 3.0%. Comparison of the results of the present HPLC method with those of gas chromatography, gave very good correlations for all fatty acids in human plasma.