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The use of bond‐elut for the estimation of serum bile pigments bonded covalently to albumin
Author(s) -
McKavanagh S. M.,
Billing B. H.
Publication year - 1987
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130020205
Subject(s) - chemistry , chromatography , covalent bond , elution , albumin , bilirubin , high performance liquid chromatography , bile pigments , conjugated system , pigment , acetonitrile , organic chemistry , biochemistry , medicine , gastroenterology , polymer
A simple and precise method has been devised for the quantitation of biliprotein (δ‐bilirubin or albumin bound bilirubin) in serum. In the presence of caffeine/benzoate, Bond‐Elut (C8, 200 mg) extracts unconjugated and conjugated bilirubin but not pigments that are covalently bonded to albumin which pass through the column and can be quantitated by a standard diazo method. Following elution from the Bond‐Elut column with methanol‐acetonitrile (50: 50, v/v) unconjugated and conjugated bilirubin can be quantitated either as total pigments or individually by HPLC.