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Automated and routine liquid chromatographic analysis of antiepileptic drugs
Author(s) -
Pang ChiPui,
Ho KwokChu,
Cheung ChiKeung
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010409
Subject(s) - chromatography , primidone , chemistry , carbamazepine , clonazepam , phenytoin , therapeutic drug monitoring , extraction (chemistry) , antiepileptic drug , immunoassay , accuracy and precision , drug , pharmacology , epilepsy , neuroscience , antibody , immunology , biology , statistics , mathematics , medicine
We describe an optimized automated liquid Chromatographic method for simultaneous measurement of primidone, phenobarbitone, phenytoin, carbamazepine and clonazepam. A Waters Tri‐Module automation system is used and it provides direct read‐out of results after chromatography. A one‐step extraction with ethyl acetate is used to extract the drugs from 100 μL serum samples. We use an isocratic mobile phase and monitor the column effluent at 210 nm. Drug levels as low as 5 μmol/L can be detected. The within‐run CV's range from 1.4 to 2.7%, and the between‐run CV's range from 5.2 to 6.1%. Analytical recovery is in the range from 94–108%. The method compares favourably with the enzyme multiple immunoassay technique for routine antiepileptic drugs monitoring, in accuracy, efficiency and cost‐effectiveness.