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HPLC determination of orthophosphate coexisting with large amounts of organophosphates in biological samples
Author(s) -
Koshiishi Ichiro,
Imanari Toshio
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010408
Subject(s) - chemistry , chromatography , ammonium acetate , high performance liquid chromatography , acetonitrile , ammonium molybdate , sulfuric acid , hydrolysis , molybdate , ammonium carbonate , buffer (optical fiber) , nuclear chemistry , inorganic chemistry , organic chemistry , raw material , telecommunications , computer science
A simple and accurate HPLC method for the determination of Orthophosphate in the presence of large amounts of organophosphates is described. The method is based on the formation and separation of the molybdenum Orthophosphate complex. In order to prevent the hydrolysis of organophosphates, the sample was deproteinized with silicotungstate in acetate buffer (pH 4.0) under ice‐cooling and then treated with ammonium molybdate in maleate buffer (pH 7.0). The sample was injected onto Styragel 60 Å column (5 mm ID × 100 mm) with 38% (v/v) acetonitrile containing 0.3 M sulfuric acid as eluent. Detection was at 310 nm. The method was applied to the determination of Orthophosphate in liver, kidney, spleen and mouse blood.