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Simple determination of forphenicinol in human plasma and erythrocytes by HPLC with native fluorescence detection
Author(s) -
Kai Masaaki,
Tamura Kazuhiko,
Ohno Masahiro,
Ohkura Yosuke
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010402
Subject(s) - chemistry , chromatography , fluorescence , elution , high performance liquid chromatography , human plasma , detection limit , gradient elution , blood plasma , biochemistry , physics , quantum mechanics
A high performance liquid chromatographic method is described for monitoring forphenicinol, a possible therapeutic drug for cancer and muscular dystrophy, in human plasma and erythrocytes. Forphenicinol in the deproteinized samples was separated from interfering biogenic substances on an aminopropyl‐bonded silica (Unicil NH 2 ) column within 10 minutes with isocratic elution, and determined with fluorescence detection. The detection limits for forphenicinol in plasma and erythrocytes are 65 pmol (12.8 ng)/ml and 160 pmol (31.5 ng)/ml, respectively, corresponding to 2 pmol each in a 100 μl injection volume. The method is very simple, and sensitive enough to permit the quantification of forphenicinol in the blood samples from man dosed with forphenicinol.