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Measurement of penbutolol and 4‐hydroxypenbutolol in plasma or serum by HPLC
Author(s) -
Bhamra R. K.,
Flanagan R. J.,
Holt D. W.
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010309
Subject(s) - chemistry , chromatography , detection limit , ether , high performance liquid chromatography , methanol , octane , centrifugation , column chromatography , silica gel , organic chemistry
A simple HPLC method for penbutolol and 4‐hydroxypenbutolol assay has been developed. Plasma or serum (200 μl) is vortex‐mixed (30 s) with Tris solution (2 M , pH 10.6) containing an internal standard (50 μl) and methyl t‐butyl ether (200 μl). After centrifugation, the extract (100 μl) is analysed using an unmodified silica column (250 × 5 mm ID) and iso‐octane–methanol–methyl t‐butyl ether (55:25:20) containing ammonium perchlorate (10 m M , pH 5.7) as eluent and with fluorescence detection. No interference has been encountered and the limit of accurate measurement for both compounds is 5 μg/l.