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A simple quantitative HPLC assay for ifosfamide in biological fluids
Author(s) -
Margison Jennifer M.,
Wilkinson P. M.,
Cerny T.,
Thatcher N.
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010303
Subject(s) - chemistry , chromatography , ifosfamide , biological fluids , simple (philosophy) , high performance liquid chromatography , medicine , surgery , chemotherapy , etoposide , philosophy , epistemology
A high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height × detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 ng/ml. Authentic 14 C‐labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentration‐time curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.