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Early identification of isovaleric aciduria using photodiode array detection for liquid chromatographic profiling of urinary carboxylic acids
Author(s) -
Buchanan D. N.,
Thoene J. G.
Publication year - 1986
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130010109
Subject(s) - chemistry , chromatography , derivatization , urine , absorbance , trimethylsilyl , chromatographic separation , carboxylic acid , solvent , residue (chemistry) , high performance liquid chromatography , organic chemistry , biochemistry
The 190 nm high performance liquid chromatographic photodiode array profiling of the urinary carboxylic acids of the first urine of a newborn affected with isovaleric aciduria afforded an abnormal peak at 27.8 min. This peak was greatly increased in the carboxylic acid profiling of the 14 h urine sample from the same infant. Isolation of this peak by fraction collecting; solvent extraction of the eluent; trimethylsilyl derivatization of the residue and gas chromatographic/mass spectrometric analysis identified the compound as isovalerylglycine. Correlation of the 190 nm absorbance of isovalerylglycine ( y ) with concentration ( x ) afforded a least squares curve: y = 476.4 x – 13.72 ( r = 0.99) run‐to‐run variation 6.92%; day‐to‐day variation 8.88%; with a minimum detectable concentration of 25 μg/ml.