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Application of liquid chromatography–electrospray ionization time‐of‐flight mass spectrometry for analysis and quality control of compound Danshen preparations
Author(s) -
Cao Jun,
Qi LianWen,
Chen Jun,
Yi Ling,
Li Ping,
Ren MeiTing,
Li YanJing
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130
Subject(s) - chemistry , chromatography , formic acid , electrospray ionization , mass spectrometry , analyte , time of flight mass spectrometry , electrospray , acetonitrile , aqueous solution , elution , detection limit , ionization , ion , organic chemistry
A liquid chromatography coupled with electrospray ionization time‐of‐flight mass spectrometry (LC‐ESI‐TOF‐MS) method has been developed to evaluate the quality of three formulas of compound Danshen preparations (CDPs), through a simultaneous determination of 22 marker constituents (nine major phenolic acids, eight major saponins and five major diterpenoids). Optimum separations were obtained with a Zorbax C 18 column, using a gradient elution with 0.1% aqueous formic acid and acetonitrile. Limits of detection and quantification were in ranges of 1.58–10.10 and 4.85–28.56 ng/mL. All calibration curves showed good linear regression ( r 2 >p;0.9900) within the test range, and the recoveries were between 78.4 and 103.1% for all analytes. The assay was successfully utilized to analyze the 22 marker components in 26 samples. The overall results demonstrated that this method is sensitive, accurate and reliable for the quality control of CDPs. Copyright © 2008 John Wiley & Sons, Ltd.