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Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis
Author(s) -
Ye Nengsheng,
Zhu Ruohua,
Gu Xuexin,
Zou Hong
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.112
Subject(s) - anisodamine , chemistry , chromatography , scopolamine , atropine , capillary electrophoresis , tropane , capillary action , pharmacology , stereochemistry , anesthesia , medicine , materials science , composite material
Abstract A capillary electrophoresis method was developed for the separation and determination of tropane alkaloids in Flos daturae plants. Separation was performed on a fused silica capillary(42.1 cm × 50 µm i.d.) at an applied voltage of 20 kV. Scopolamine, atropine and anisodamine were well separated in the buffer of 50 mmol/L phosphate buffer (pH 5.0) containing 20% (v/v) tetrahydrofuran (THF). Beer's law was obeyed in the range of concentration of 2.4–21.8 µg/mL for scopolamine, 4.0–36.0 µg/mL for atropine and 2.6–23.7 µg/mL for anisodamine, respectively, and the correlation coefficients were over 0.999 ( n = 6). The developed method was applied for the analysis of herb samples. Copyright © 2001 John Wiley & Sons, Ltd.