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Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens
Author(s) -
Xu Yi Ping,
Liu Li Qiang,
Li Qiu Shen,
Peng Chi Fang,
Chen Wei,
Xu Chuan Lai
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1115
Subject(s) - chemistry , chromatography , detection limit , analyte , derivatization , metabolite , nitrofuran , urine , polyclonal antibodies , antibody , high performance liquid chromatography , biochemistry , immunology , genetics , biology
A rapid immunochromatographic assay was developed and validated for detection of 1‐aminohydantoin (AHD) in urine specimens. Colloidal gold‐labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative‐conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL −1 and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme‐linked immunosorbent assay. The semi‐quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues. Copyright © 2008 John Wiley & Sons, Ltd.