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Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis
Author(s) -
Elbashir Abdalla A.,
Saad Bahruddin,
Ali Abdussalam Salhin Mohamed,
Saleh Muhammad Idiris,
AboulEnein Hassan Y.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1113
Subject(s) - capillary electrophoresis , chemistry , chromatography , primaquine , capillary action , enantiomer , impurity , tris , electrolyte , derivatization , analytical chemistry (journal) , mass spectrometry , stereochemistry , electrode , biochemistry , materials science , organic chemistry , chloroquine , malaria , immunology , composite material , biology
A capillary electrophoretic (CE) method for the baseline separation of the enantiomers of primaquine diphosphate (PQ) and quinocide (QC) (a major contaminant) in pharmaceutical formulations is proposed. Both components were separated under the following conditions: 50 m m tris phosphate buffer (pH 3.0) containing 15 m m hydroxypropyl‐ γ ‐cyclodextrin (HP‐ γ ‐CD) as background electrolyte; applied voltage, 16 kV; capillary temperature, 25°C; detection wavelength, 254 nm; hydrostatic injection, 10 s. The separations were conducted using a 35 cm length and 50 µm i.d. uncoated fused silica capillary column. Under the optimized conditions, the components were successfully separated in about 5 min. Intraday precision of migration time and corrected peak areas when expressed as relative standard deviation ranged from 0.17 to 0.45 and 2.60 to 3.94%, respectively, while the interday precision ranged from 2.59 to 4.20 and 3.15 to 4.21%, respectively. After the validation exercise, the proposed method was applied for the determination of QC impurity in PQ formulations. Copyright © 2008 John Wiley & Sons, Ltd.