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Method validation for measurement of hair nicotine level in nonsmokers
Author(s) -
Kim Sung Roul,
Wipfli Heather,
AvilaTang Erika,
Samet Jonathan M.,
Breysse Patrick N.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1110
Subject(s) - isotope dilution , chemistry , nicotine , chromatography , dilution , extraction (chemistry) , matrix (chemical analysis) , gas chromatography–mass spectrometry , secondhand smoke , gas chromatography , environmental chemistry , mass spectrometry , environmental health , medicine , physics , thermodynamics
The development of strategies to address the growing worldwide burden of exposure to secondhand smoke (SHS) would be facilitated by sensitive and accurate methods for assessing SHS exposure. Hair provides a readily available matrix for assessing biomarkers of typical SHS exposure. We developed and applied an optimized analytical method using an isotope dilution gas chromatography–mass spectrometry (GC/MS) for hair nicotine measurement. The utility of this optimized method is illustrated by presenting data on SHS exposure of women and children from 31 countries. Using this isotope dilution method with spiked samples (3.3 ng/mg), we found that the greatest hair nicotine extraction efficiency was obtained with a 60 min shaking time. In the field study ( n = 2400), a positive association was evident between hair nicotine concentrations from nonsmokers and higher numbers of cigarettes smoked per day in a household. Copyright © 2008 John Wiley & Sons, Ltd.