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A proteomics study on human breast cancer cell lines by fluorogenic derivatization–liquid chromatography/tandem mass spectrometry
Author(s) -
Imai Kazuhiro,
Ichibangase Tomoko,
Saitoh Ryoichi,
Hoshikawa Yutaka
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1102
Subject(s) - chemistry , derivatization , tropomyosin , proteome , proteomics , tandem mass spectrometry , breast cancer , cancer cell , human breast , mass spectrometry , chromatography , liquid chromatography–mass spectrometry , cancer , biochemistry , actin , biology , genetics , gene
Although several molecular markers for human breast cancer exist, their versatility is limited. Here we demonstrate, through a differential proteome analysis utilizing the fluorogenic derivatization–liquid chromatography/tandem mass spectrometry (FD‐LC‐MS/MS) method between seven cancer cells and one normal cell, that the presence of cooperatively expressed annexin‐2 and galectin‐1 without tropomyosin‐1 in a tissue could be used to diagnose metastatic breast cancer. Interestingly, in a metastatic cancer cell, the expression of the former two together with highly expressed cofilin‐1 activates the Rho signal pathway to aggressively form disorganized actin filaments. Despite the excess expression of annexin‐2 and galectin‐1 in the normal cell, the highly expressed tropomyosin‐1 counteracted the activity of cofilin‐1 and stabilized the filaments, resulting in the restoration of the disorganization. This phenomenon suggests that enhancement of tropomyosin‐1 should be used as therapy for metastatic breast cancer. Copyright © 2008 John Wiley & Sons, Ltd.