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Determination of serotonin, melatonin and metabolites in gastrointestinal tissue using high‐performance liquid chromatography with electrochemical detection
Author(s) -
Chau Rosanna M. W.,
Patel Bhavik Anil
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1100
Subject(s) - chemistry , chromatography , melatonin , perchloric acid , detection limit , analyte , gastrointestinal tract , high performance liquid chromatography , serotonin , sulfonic acid , quantitative analysis (chemistry) , acetonitrile , sample preparation , biochemistry , endocrinology , organic chemistry , medicine , receptor
In this paper we show a simple isocratic chromatographic method for the detection of serotonin and its precursors and metabolites from various types of gastrointestinal tissue. The paper measures for the first time basal measurements of melatonin in the gastrointestinal tract, which has recently been shown to be released from the musosal lining of the gut. Tissue samples were stable following sample preparation in either 0.1 m perchloric acid or mobile phase. Analysis was carried out using a mobile phase consisting of 10% acetonitrile–90% acetate acid buffer pH 4.0 with 2 m m decane–sulfonic acid sodium salt at a column temperature of 50°C. Electrochemical detection was utilized at a potential of +850 mV vs Ag/AgCl reference electrode at 10 µA full‐scale deflection. The detection limit of 5‐HT and melatonin was 241 and 308 nm respectively for a 10 µL injection. As a result of the method optimization, total analysis was reduced to 30 min. Accurate responses of the tissue samples following sample preparation could be obtained following a week after storage at –80°C. This method is capable of preparing and analysing of samples from all regions of the gastrointestinal tract. Copyright © 2008 John Wiley & Sons, Ltd.