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High‐performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect fluorescence detection
Author(s) -
AlKindy Salma M.,
Miller James N.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1098
Subject(s) - chemistry , detection limit , chromatography , naphthalene , fluorophore , high performance liquid chromatography , fluorescence , coumarin , extraction (chemistry) , calibration curve , solid phase extraction , carboxylic acid , quenching (fluorescence) , organic chemistry , physics , quantum mechanics
A high‐performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre‐concentrated using solid‐phase extraction and well resolved on a C 18 column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8‐tetrahydronaphthol (5,6,7,8‐THN‐1‐OH), 7‐amino‐4‐methyl coumarin (Coumarin 120, COU‐120) and 3‐hydroxy‐4‐(2‐hydroxy‐4‐sulfo‐1‐naphthylazo)2‐naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 × 10 −9 to 1.1 × 10 −8 mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 × 10 −10 mol/L for 1‐nitronaphthalene and 2.1 × 10 −11 mol/L for 2‐nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%. Copyright © 2008 John Wiley & Sons, Ltd.