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A rapid and specific approach for direct measurement of donepezil concentration in human plasma by LC‐MS/MS employing solid‐phase extraction
Author(s) -
Shah Hiten J.,
Kundlik Mohan L.,
Pandya Ankit,
Prajapati Shivkumar,
Subbaiah Gunta,
Patel Chhagan N.,
Patel Dasharath M.,
Suhagiya Bhanu N.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1095
Subject(s) - chemistry , chromatography , formic acid , solid phase extraction , extraction (chemistry) , detection limit , liquid chromatography–mass spectrometry , mass spectrometry , selected reaction monitoring , tandem mass spectrometry , donepezil , medicine , dementia , disease , pathology
Abstract A selective, rapid and simple liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method is described for assay of donepezil in human plasma using escitalopram as an internal standard. Chromatographic separation was achieved on a Betabasic‐C 8 , 5 µm, 100 × 4.6 mm column using methanol:water:formic acid (90:9.97:0.03, v/v/v) as mobile phase. Detection of donepezil and internal standard was achieved by ESI MS/MS in positive ion mode using 380.20/91.10 and 325.13/262.00 transitions, respectively. The linearity over the concentration range of 0.15–50 ng/mL for donepezil was obtained and the lower limit of quantification was 0.15 ng/mL. For each level of quality control samples, inter‐day and intra‐day precisions (RSD) were ≤8.92 and 10.35% and accuracy (%RE) were ≤7.33% and 9.33%, respectively. The recovery was more than 88.50% for both donepezil and internal standard by solid‐phase extraction, eliminating evaporation and reconstitution steps. Copyright © 2008 John Wiley & Sons, Ltd.