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Automated analysis of fluvoxamine in rat plasma using a column‐switching system and ion‐pair high‐performance liquid chromatography
Author(s) -
Liu Shicheng,
Shinkai Norihiro,
Kakubari Ikuhiro,
Saitoh Hideo,
Noguchi Kenichi,
Saitoh Takashi,
Yamauchi Hitoshi
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1079
Subject(s) - chromatography , chemistry , analyte , calibration curve , high performance liquid chromatography , fluvoxamine , phosphoric acid , detection limit , elution , extraction (chemistry) , analytical chemistry (journal) , biochemistry , receptor , organic chemistry , serotonin , fluoxetine
Abstract We have established a robust, fully automated analytical method for the analysis of fluvoxamine in rat plasma using a column‐switching ion‐pair high‐performance chromatography system. The plasma sample was injected onto a precolumn packed with Shim‐pack MAYI‐ODS (50 µm), where the drug was automatically purified and enriched by on‐line solid‐phase extraction. After elution of the plasma proteins, the analyte was back‐flushed from the precolumn and then separated isocratically on a reversed‐phase C18 column (L‐column ODS) with a mobile phase (acetonitrile–0.1% phosphoric acid, 36:64, v/v) containing 2 m m sodium 1‐octanesulfonate. The analyte was monitored by a UV detector at a wavelength of 254 nm. The calibration line for fluvoxamine showed good linearity in the range of 5–5000 ng/mL ( r > 0.999) with the limit of quantification of 5 ng/mL (RSD = 6.51%). Accuracy ranged from −2.94 to 4.82%, and the within‐ and between‐day precision of the assay was better than 8% across the calibration range. The analytical sensitivity and accuracy of this assay is suitable for characterization of the pharmacokinetics of orally‐administered fluvoxamine in rats. Copyright © 2008 John Wiley & Sons, Ltd.