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Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high‐performance liquid chromatography coupled with mass spectrometry
Author(s) -
Su ShuLan,
Yu Li,
Hua YongQing,
Duan JinAo,
Deng HaiShan,
Tang YuPing,
Lu Yin,
Ding AnWei
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1070
Subject(s) - paeoniflorin , chemistry , chromatography , human umbilical vein endothelial cell , umbilical vein , decoction , high performance liquid chromatography , extraction (chemistry) , mass spectrometry , in vitro , biochemistry , traditional medicine , medicine
Abstract In this paper, a useful method for screening and analyzing the potential bioactive components in bioassay‐guided fraction (SF‐11) from Shaofu Zhuyu decoction was developed using human umbilical vein endothelial cell (HUVEC) extraction and high‐performance liquid chromatography coupled with Q‐TOF/MS spectrometry. In addition, the protective effects on HUVEC damage induced by adrenaline in vitro were also investigated. The results showed that SF‐11 significantly inhibited the endothelin (ET) release and reversed the NO secretion of HUVEC ( p < 0.05), and promoted the PGI 2 release of HUVEC ( p < 0.05). Two effective components, paeoniflorin and typhaneoside, from SF‐11 were screened and identified using live cell extract and HPLC coupled with Q‐TOF/MS spectrometry. The compounds, paeoniflorin and typhaneoside, showed significantly inhibiting effects on the ET release and reversing of NO secretion of HUVEC ( p < 0.05), with similar effects to SF‐11, and promoting the PGI 2 release of HUVEC at the concentration of 0.208 and 0.013 µmol/mL, respectively ( p < 0.05). These data indicated that the method of live cell extraction coupled with HPLC‐MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCMs. Copyright © 2008 John Wiley & Sons, Ltd.