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Analysis of urinary aromatic acids by liquid chromatography tandem mass spectrometry
Author(s) -
Crow Brian,
Bishop Michael,
Paliakov Ekaterina,
Norton Dean,
George Joe,
Bralley J. Alexander
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1064
Subject(s) - chemistry , chromatography , analyte , tandem mass spectrometry , mass spectrometry , accuracy and precision , chromatographic separation , liquid chromatography–mass spectrometry , sample preparation , high performance liquid chromatography , tandem , dilution , statistics , mathematics , materials science , physics , composite material , thermodynamics
The separation and detection of 11 urinary aromatic acids was developed using HPLC‐MS/MS. The method features a simple sample preparation involving a single‐step dilution with internal standard and a rapid 8 min chromatographic separation. The accuracy was evaluated by the recovery of known spikes between 87 and 110%. Inter‐ and intra‐assay precision (CV) was below 11% in all cases and the analytes were observed to be stable for up to 8 weeks when stored at −20°C. The method was validated based upon linearity, accuracy, precision and stability and was used to establish reference intervals for children and adults. Copyright © 2008 John Wiley & Sons, Ltd.