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Subunit structure of glycinin and its molecular species based on RP‐HPLC, gel electrophoresis and SEC studies
Author(s) -
Bhushan Ravi,
Arya Upasana
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1063
Subject(s) - chemistry , chromatography , gel electrophoresis , protein subunit , high performance liquid chromatography , size exclusion chromatography , electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , biochemistry , enzyme , gene
Glycinin and its molecular species (glycinin I and glycinin II) were separated and isolated. The number and kind of subunits of glycinin, glycinin I and glycinin II were determined. Studies were carried out under different experimental conditions using slab gel electrophoresis, size‐exclusion chromatography and reversed‐phase high performance liquid chromatography. Gel electrophoresis was done using both continuous and discontinuous system and under varying concentrations of resolving gel. In addition, the subunits were separated by reversed phase using gradient program. Glycinin and glycinin II were found to have 12 subunits each while glycinin I showed six subunits. Molecular weight and weight ratio in each case were also determined. Copyright © 2008 John Wiley & Sons, Ltd.