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Mixed micellar liquid chromatography methods: modelling quantitative retention–activity relationships of angiotensin converting enzyme inhibitors
Author(s) -
Wu Liping,
Cui Yan,
Xiong Meijin,
Wang Shurong,
Chen Cong,
Ye Liming
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1053
Subject(s) - chemistry , chromatography , micellar liquid chromatography , enzyme , micelle , phase (matter) , biochemistry , organic chemistry , aqueous solution
The capability of biopartitioning micellar chromatography (BMC), using pure Brij35 solution and mixed micellar system of Brij35–SDS (85:15) as mobile phase, to describe and estimate bioactivities of angiotensin converting enzyme inhibitors at different pH has been studied. Quantitative retention–activity relationships (QRAR) in BMC were investigated for these compounds. The obtained BMC Brij35–SDS –QRAR models were compared with the traditional BMC Brij35 –QRAR, and better statistically models were obtained using Brij35‐SDS retention data. The superiority of BMC Brij35–SDS –QRAR is due to the fact that the mixed micellar mobile phase can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged. Copyright © 2008 John Wiley & Sons, Ltd.