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Improved method to determine succinylacetone in dried blood spots for diagnosis of tyrosinemia type 1 using UPLC‐MS/MS
Author(s) -
AlDirbashi Osama Y.,
Rashed Mohamed S.,
Jacob Minnie,
AlAhaideb Lujane Y.,
AlAmoudi Mohamed,
Rahbeeni Zuhair,
AlSayed Moeen M.,
AlHassnan Zuhair,
AlOwain Mohamed,
AlZeidan Hamad
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1049
Subject(s) - chemistry , chromatography , dried blood , tyrosinemia , spots , biochemistry , tyrosine
We describe an improved diagnostic method for tyrosinemia type 1 based on quantifying succinylacetone in dried blood spots by ultra‐performance liquid chromatography tandem mass spectrometry. Succinylacetone extracted from a single 3/16 inch disk of specimen collection paper containing a dried blood spot was derivatized with dansylhydrazine, separated on an Acquity UPLC BEH C 18 column (2.1 × 50 mm, 1.7 µm) and detected by electrospray ionization tandem mass spectrometry. Succinylacetone derivative eluted at 0.6 min with a complete run time of 1 min. Using a 13 C 4 labeled succinylacetone as an internal standard, the calibration plot was linear up to 100 µmol/L with a detection limit (S/N = 3) of 0.2 µmol/L. Intra‐day ( n = 13) and inter‐day ( n = 10) variations were better than 10%. The cutoff level of succinylacetone in dried blood spots from healthy infants obtained by the current method was 0.63 µmol/L ( n = 151). In dried blood spots from patients with established tyrosinemia type 1 ( n = 11), concentration of succinylacetone was 6.4–30.8 µmol/L. Copyright © 2008 John Wiley & Sons, Ltd.