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LC‐MS/MS method for the determination of cynandione A in rat plasma and tissues
Author(s) -
Zhang Yaping,
Shan Lei,
Xu Wen,
Yang Ming,
Zhang Weidong,
Li Haiyun
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1044
Subject(s) - chemistry , chromatography , calibration curve , formic acid , selected reaction monitoring , electrospray ionization , honokiol , detection limit , mass spectrometry , extraction (chemistry) , electrospray , ethyl acetate , analytical chemistry (journal) , tandem mass spectrometry
A rapid and sensitive method using liquid chromatography–tandem mass spectroscopy (LC‐MS/MS) was developed and validated for the quantitative determination of cynandione A in rat plasma and tissues. The plasma samples were pretreated by liquid–liquid extraction with ethyl acetate after the internal standard (honokiol) had been spiked. The tissue samples were homogenized with physiological saline and treated further like the plasma samples. The separation was performed using a Zorbax SB‐C 18 column (3.5 µm, 2.1 × 100 mm) and a C 18 guard column (5 µm, 4.0 × 2.0 mm) with an isocratic mobile phase consisting of methanol–0.1% formic acid (78:22, v/v) at a flow rate of 0.2 mL/min. The Agilent G6410A triple quadrupole LC/MS system was operated under the multiple‐reaction monitoring mode using the electrospray ionization technique in negative mode. The nominal retention times for cynandione A and honokiol were 1.41 and 2.63 min, respectively. The method was validated within the concentration range 0.2–1000 ng/mL in plasma and homogenized tissue for cynandione A, and the calibration curves were linear with correlation coefficients >0.992. The lower limit of quantification of cynandione A was 0.2 ng/mL. The intra‐day and inter‐day precision and accuracy of the assay in plasma were less than 14.4%, while the intra‐day and inter‐day precision and accuracy of the assay in tissue homogenate were less than 14.2%. This method proved to be suitable for study of pharmacokinetics and tissue distribution of cynandione A in rat. Copyright © 2008 John Wiley & Sons, Ltd.