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A sensitive method for determination of salvianolic acid A in rat plasma using liquid chromatography/tandem mass spectrometry
Author(s) -
Pei Lixia,
Bao Yuanwu,
Wang Haidi,
Yang Fan,
Xu Bei,
Wang Shoubao,
Yang Xiuying,
Du Guanhua
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1043
Subject(s) - chemistry , chromatography , liquid chromatography–mass spectrometry , selected reaction monitoring , pharmacokinetics , tandem mass spectrometry , extraction (chemistry) , mass spectrometry , detection limit , pharmacology , medicine
Salvianolic acid A (SAA), a major effective constituent of Salvia miltiorrhizas , is widely used in traditional Chinese medicine. A sensitive rapid analytical method was established and validated for SAA in rat plasma, which was further applied to assess the pharmacokinetics of SAA in rats receiving a single oral dose of SAA. The method used liquid chromatography tandem mass spectrometry in multiple reaction monitoring mode with chloramphenicol as the internal standard. A simple liquid–liquid extraction based on ethyl acetate was employed. The combination of a simple sample cleanup and short chromatographic run time (3 min) increased the throughput of the method substantially. The method was validated over the range 1.4–1000 ng/mL with a correlation coefficient >0.99. The lower limit of quantification was 1.4 ng/mL for SAA in plasma. Intra‐ and inter‐day accuracies for SAA were 95–113 and 98–107%, and the inter‐day precision was less than 12%. This method is more sensitive and faster than previous methods. After a single oral dose of 100 mg/kg of SAA, the mean peak plasma concentration ( C max ) of SAA was 318 ng/mL at 0.5 h, the area under the plasma concentration–time curve (AUC 0–12 h ) was 698 ± 129 ng·h/mL, and the elimination half‐life ( T 1/2 ) was 3.29 h. Copyright © 2008 John Wiley & Sons, Ltd.