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Ultra‐fast chromatographic micro‐assay for quantification of diphenidol in plasma: application in an oral multi‐dose switchability trial
Author(s) -
MarcelínJiménez Gabriel,
MoralesMartínez Miriam,
ÁngelesMoreno Alionka P.,
MendozaMorales Luís
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1037
Subject(s) - chromatography , chemistry , bioequivalence , electrospray , protein precipitation , pharmacokinetics , mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , selected reaction monitoring , pharmacology , medicine
Abstract Pharmacokinetics of diphenidol (DPN) is limited due to the lack of analytical methodology. Here, a micro‐assay for DPN quantification was developed, by coupling ultra‐performance liquid chromatography with tandem mass spectrometry. The procedure involved plasma precipitation and injection of supernatant into UPLC with an Acquity™ C 18 column. Detection was in positive electrospray, following transitions of m/z 310.3 → 292.3 and m/z 275.3 → 230.2 for DPN and chlorphenamine (internal standard), respectively. The method was linear with a range of 4–400 ng/mL, and a 2 min run time. This method was applied in a switchability trial, where both formulations of DPN were bioequivalent. Copyright © 2008 John Wiley & Sons, Ltd.