Ultra‐fast chromatographic micro‐assay for quantification of diphenidol in plasma: application in an oral multi‐dose switchability trial | Zendy

MarcelínJiménez Gabriel | Zendy; MoralesMartínez Miriam | Zendy; ÁngelesMoreno Alionka P. | Zendy; MendozaMorales Luís | Zendy

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Ultra‐fast chromatographic micro‐assay for quantification of diphenidol in plasma: application in an oral multi‐dose switchability trial

Author(s) -

MarcelínJiménez Gabriel,

MoralesMartínez Miriam,

ÁngelesMoreno Alionka P.,

MendozaMorales Luís

Publication year - 2008

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1037

Subject(s) - chromatography , chemistry , bioequivalence , electrospray , protein precipitation , pharmacokinetics , mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , selected reaction monitoring , pharmacology , medicine

Pharmacokinetics of diphenidol (DPN) is limited due to the lack of analytical methodology. Here, a micro‐assay for DPN quantification was developed, by coupling ultra‐performance liquid chromatography with tandem mass spectrometry. The procedure involved plasma precipitation and injection of supernatant into UPLC with an Acquity™ C 18 column. Detection was in positive electrospray, following transitions of m/z 310.3 → 292.3 and m/z 275.3 → 230.2 for DPN and chlorphenamine (internal standard), respectively. The method was linear with a range of 4–400 ng/mL, and a 2 min run time. This method was applied in a switchability trial, where both formulations of DPN were bioequivalent. Copyright © 2008 John Wiley & Sons, Ltd.

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