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A high‐performance liquid chromatographic method for determination of scopolin in rat plasma: application to pharmacokinetic studies
Author(s) -
Xia YuFeng,
Dai Yue,
Wang Qiang,
Cai Fei
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1036
Subject(s) - chromatography , chemistry , high performance liquid chromatography , protein precipitation , calibration curve , distilled water , detection limit , methanol , acetic acid , biochemistry , organic chemistry
An analytical method based on high‐performance liquid chromatographic (HPLC) with ultraviolet (UV) detection was developed for determination of scopolin in rat plasma using aesculin as internal standard (IS). After protein precipitation of plasma sample with methanol, the supernatant was directly injected and analyzed. Chromatographic separation was achieved on a C 18 column using methanol and distilled water (22:78, v/v) containing 0.2% (v/v) glacial acetic acid as mobile phase with a column temperature of 30°C. The UV detector was set at 338 nm. The calibration curve was linear over the range of 0.105–13.125 µg/mL with a correlation coefficient of 0.9998. The retention times of aesculin and scopolin were 10.4 and 12.8 min, respectively. The recoveries for plasma samples of 0.105, 4.725 and 13.125 µg/mL were 91.08, 95.30 and 96.10%, respectively. The RSD of intra‐ and inter‐day assay variations was less than 7.35%. The lower limit of detection was 0.03 µg/mL .This HPLC assay is a simple, sensitive and accurate and was successfully applied to the pharmacokinetic study of scopolin in rats. Copyright © 2008 John Wiley & Sons, Ltd.