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Identification of differentially expressed proteins in the heart of translationally controlled tumor protein over‐expressing transgenic mice
Author(s) -
Cheon Myeong Sook,
Suh Jaekyung,
Kim MinJeong,
Kim Seong Hwan,
Lee Kyunglim
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1029
Subject(s) - genetically modified mouse , western blot , heat shock protein , transgene , proteomics , chemistry , metabolism , peroxiredoxin , microbiology and biotechnology , hsp70 , biochemistry , protein subunit , biology , gene , peroxidase , enzyme
We previously reported that transgenic (TG) mice over‐expressing translationally controlled tumor protein (TCTP) developed systemic arterial hypertension at about 6 weeks after birth. In the present study, we identified, using proteomics technologies, 24 other proteins that were differentially expressed in the heart of TCTP over‐expressing TG mice. These 24 proteins are involved in a variety of biological processes such as reactive oxygen species metabolism, cytoskeleton organization, fatty acid metabolism, amino acid metabolism and energy metabolism. We determined protein expression levels of the peroxiredoxin (Prx)2, Prx3, myosin light chain 1, stress protein (heat shock protein) 25K, and T‐complex protein 1 alpha subunit by western blot analysis. Over‐expression of TCTP probably regulates the expression of other proteins which play a pivotal role in a variety of cellular functions in TCTP over‐expressing TG mice. Copyright © 2008 John Wiley & Sons, Ltd.