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Optimization, validation and application of a method for the determination of trichloroethylene in rat plasma by headspace solid‐phase microextraction gas chromatography mass spectrometry
Author(s) -
Liu Yongzhen,
Muralidhara Srinivasa,
Bruckner James V.,
Bartlett Michael G.
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1016
Subject(s) - chemistry , trichloroethylene , chromatography , detection limit , solid phase microextraction , gas chromatography–mass spectrometry , mass spectrometry , gas chromatography , tetrachloroethylene , solvent , extraction (chemistry) , environmental chemistry , organic chemistry
Trichloroethylene (TCE) is a small halogenated compound that has been used extensively as a metal degreaser and a solvent for the past 100 years. As a result of its widespread use, TCE can be found in the groundwater of about one‐third of the hazardous waste sites on the United States Environmental Protection Agency's National Priorities List. Human exposure to TCE in the environmental media is of concern because TCE has been found to be carcinogenic in laboratory animals. This paper describes the development and validation of a HS‐SPME‐GC/MS method for determination of TCE in rat plasma. The effects of different parameters such as sample volume, extraction and desorption conditions, fiber positions and salt addition were investigated and optimized. The method is rapid, simple, sensitive and requires a very small sample volume. The lower limit of quantitation was 0.25 ng/mL and correlation coefficient ( r 2 ) values for the linear range of 0.25–100 ng/mL were 0.996 or greater. The precision and accuracy for intra‐day and inter‐day were better than 8.0%. This validated method was successfully applied to study the toxicokinetic behavior of TCE following low levels of oral administration. Copyright © 2008 John Wiley & Sons, Ltd.