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Comparative analysis on microbial and rat metabolism of ginsenoside Rb 1 by high‐performance liquid chromatography coupled with tandem mass spectrometry
Author(s) -
Chen Guangtong,
Yang Min,
Song Yan,
Lu Zhiqiang,
Zhang Jinqiang,
Huang Huilian,
Guan Shuhong,
Wu Lijun,
Guo Dean
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1001
Subject(s) - chemistry , ginsenoside , chromatography , tandem mass spectrometry , metabolism , saponin , protopanaxadiol , in vivo , liquid chromatography–mass spectrometry , high performance liquid chromatography , mass spectrometry , metabolite , biochemistry , ginseng , biology , medicine , alternative medicine , microbiology and biotechnology , pathology
Ginsenoside Rb 1 is an active protopanaxadiol saponin from Panax species. In order to compare the similarities and differences of microbial and mammalian metabolisms of ginsenoside Rb 1 , the microbial transformation by Acremonium strictum and metabolism in rats were comparatively studied. Microbial transformation of ginsenoside Rb 1 by Acremonium strictum AS 3.2058 resulted in the formation of eight metabolites. Ten metabolites (M1–M10) were detected from the in vivo study in rats and eight of them were identified as the same compounds as those obtained from microbial metabolism by liquid chromatography–tandem mass spectrometry analysis and comparison with reference standards obtained from microbial metabolism. Their structures were identified as ginsenoside Rd, gypenoside XVII, 20( S )‐ginsenoside Rg 3 , 20( R )‐ginsenoside Rg 3 , ginsenoside F 2 , compound K, 12 β ‐hydroxydammar‐3‐one‐20( S ) ‐O‐β ‐d‐ glucopyranoside, and 25‐hydroxyl‐( E )‐20(22)‐ene‐ginsenoside Rg 3 , respectively. The structures of the additional two metabolites were tentatively characterized as 20(22),24‐diene‐ginsenoside Rg 3 and 25‐hydroxyginsenoside Rd by HPLC‐MS/MS analysis. M7–M10 are the first four reported metabolites in vivo . The time course of rat metabolism of ginsenoside Rb 1 was also investigated. Copyright © 2008 John Wiley & Sons, Ltd.