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Using restriction mapping to teach basic skills in the molecular biology lab
Author(s) -
Walsh Lauren,
Shaker Elizabeth,
De Stasio Elizabeth A.
Publication year - 2007
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.45
Subject(s) - restriction enzyme , restriction digest , computational biology , restriction map , agarose gel electrophoresis , biology , gel electrophoresis , restriction fragment , restriction site , dna , plasmid , computer science , microbiology and biotechnology , genetics
Digestion of DNA with restriction enzymes, calculation of volumes and concentrations of reagents for reactions, and the separation of DNA fragments by agarose gel electrophoresis are common molecular biology techniques that are best taught through repetition. The following open‐ended, investigative laboratory exercise in plasmid restriction mapping allows students to gain technical expertise while simultaneously exploring the utility of gel electrophoresis and restriction mapping. Because of its interpretive nature, this project also provides data suitable for a written report, and can thus be used to reinforce lessons on figure presentation and science writing skills.