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A simple colorimetric experiment using mammalian cell culture to study metabolism
Author(s) -
Sharma Kanika,
Fizet Kiel J.,
Montgomery Kathlyene R.,
Smeltzer Nathan A.,
Sikorski Madison H.,
Brown Kennedy G.,
Beyke Brandon J.,
Burkhart Ryan C.,
Lynn Abigail N.,
Grandinetti Giovanna
Publication year - 2020
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21457
Subject(s) - glycolysis , metabolism , oxidative phosphorylation , biochemistry , cell culture , cellular metabolism , chemistry , biology , genetics
The goal of this laboratory exercise is to give upper‐level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3‐h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand‐alone experiment. In this experiment, students are tasked with relating the acidification of cell culture medium to metabolism in order to elucidate the mechanism of action for a compound. Students can relate their experimental results to topics covered on glycolysis and oxidative phosphorylation in upper‐level biochemistry classes as well as gain valuable experience relating metabolism to drug discovery.