Premium
Separation of mononuclear cells and identification of B lymphocytes: A comprehensive experiment for medical students
Author(s) -
Li Jiao,
Gao Furong,
Jin Caixia,
Shi Xiujuan,
Zhang Chen,
Jia Song,
Xu Jie,
Lv Lixia,
Yang Wenzhuo,
Xu Lei
Publication year - 2020
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21338
Subject(s) - formative assessment , peripheral blood mononuclear cell , identification (biology) , immunocytochemistry , computer science , immunology , medical education , computational biology , medicine , biology , pathology , psychology , mathematics education , biochemistry , botany , in vitro
Abstract In teaching of experiments for medical students, by using the isolation of mononuclear cells and identification of B lymphocytes, an experiment was developed that integrated biochemistry, cytology, and immunology techniques, from which the students performed a series of operations including spleen separation, isolating mononuclear cells, and identifying B lymphocytes. From the immunocytochemistry detection, we included advanced equipment—a flow cytometer—in the experiment, which detected the B lymphocytes more accurately. Moreover, we applied modern information technology to the teaching of experiments by using an internet study platform, micro‐lectures, and adopting “formative teaching and evaluation.” This lab practice aims to increase the ability of medical students to solve problems using flexible scientific methods, to initiate them thinking about clinical and research application.