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Implementation of research on potential drug target cloning and characterization in a biochemistry laboratory
Author(s) -
Wu Chun,
McCune Tyler S.
Publication year - 2019
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21319
Subject(s) - cloning (programming) , computational biology , biochemistry , drug discovery , chemistry , biology , computer science , bioinformatics , programming language
An approach for incorporating preliminary drug discovery research into a biochemistry laboratory is described. During a total of 42 hr (one 3‐hr laboratory section a week for 14 weeks), students were exposed to bioinformatics; molecular cloning; protein expression, purification, and characterization; and enzymatic kinetic assays. This research‐oriented laboratory not only includes the standard elements of common undergraduate biochemistry laboratory manuals but also emphasizes the logical connections among biochemistry laboratory techniques. Moreover, this approach exposed students to laboratory research and the concept of drug discovery.