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User‐friendly tool kits for protein gel electrophoresis techniques: A training program for high school students
Author(s) -
Maurye Praveen,
Basu Arpita,
Sen Sohini,
Biswas Jayanta Kumar,
Bandyopadhyay Tapas Kumar,
Naskar Malay
Publication year - 2018
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21172
Subject(s) - computer science , polyacrylamide gel electrophoresis , chromatography , chemistry , biochemistry , enzyme
Recent advancements in biochemical sciences have helped the researchers to explore the molecular logic of life inclusive of its multifarious expressions and explain many facts about the structure and functions of cellular macromolecules. Due to its simple and cost‐effective nature, polyacrylamide gel electrophoresis (PAGE) has become the most favored technique for qualitative and quantitative examination of macromolecules. Major drawbacks of such modifications are the cost and operational complexities faced by naïve students. Many interlinking laboratory equipment are needed in the school laboratories for the conduct of even simple scientific experiment. Some of these costly modern equipment are inaccessible for students of small laboratories, and their alternatives are not easily available. Many of these laboratory equipment required for routine gel electrophoresis technique can be fabricated in their simplest form using off‐the‐shelf components. A short term biochemistry training program was executed for high school students to provide them “hands‐on” training using newly modified equipment, which was proved to be an exciting way of learning biochemical gel separation techniques. © 2018 International Union of Biochemistry and Molecular Biology, 46(5):566–577, 2018.