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Chemical cross‐linking of a variety of green fluorescent proteins as Förster resonance energy transfer donors for Yukon orange fluorescent protein: A project‐based undergraduate laboratory experience
Author(s) -
Marchioretto Mira K.,
Horton Jacqueline T.,
Berstler Calvin A.,
Humphries Joseph B.,
Koloditch Isaac J.,
Voss Samuel D.,
de La Harpe Kimberly,
Hicks Barry W.,
Jefferies Latisha R.
Publication year - 2018
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21158
Subject(s) - förster resonance energy transfer , fluorescence , orange (colour) , energy transfer , chemistry , green fluorescent protein , biophysics , biology , biochemistry , physics , chemical physics , optics , food science , gene
Förster resonance energy transfer (FRET) is the basis for many techniques used in biomedical research. Due to its wide use in molecular sensing, FRET is commonly introduced in many biology, chemistry, and physics courses. While FRET is of great importance in the biophysical sciences, the complexity and difficulty of constructing FRET experiments has resulted in limited usage in undergraduate laboratory settings. Here, we present a practical undergraduate laboratory experiment for teaching FRET using a diverse set of green‐emitting fluorescent proteins (FPs) as donors for a cross‐linked Yukon orange FP. This laboratory experiment enables students to make the connection of basic lab procedures to real world applications and can be applied to molecular biology, biochemistry, physical chemistry, and biophysical laboratory courses. Published 2018. This article is a U.S. Government work and is in the public domain in the USA., 46(5):516–522, 2018.