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An integrated course based on two dimensional‐electrophoresis and mass spectrometry analysis to teach proteomic techniques for undergraduate students
Author(s) -
Sha Shanshan,
Jia Liqiu,
Xin Yi,
Yao Weili,
Ma Yufang
Publication year - 2018
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21128
Subject(s) - mass spectrometry , proteomics , computational biology , microbiology and biotechnology , gel electrophoresis , two dimensional gel electrophoresis , chemistry , biology , chromatography , biochemistry , gene
Here, we developed an integrated course based on two dimensional‐electrophoresis and spectrometry mass (2DE‐MS) technique for undergraduate students to help them learn proteomic techniques. The soluble proteins in wild type and gene knockout bacteria were separated by 2DE and the differently expressed proteins were identified by MS analysis. The proteomic data was finally confirmed by RT‐PCR detection. The separated experiments of 2DE, MS, RNA isolation, RT‐PCR, as well as essential bioinformatic analysis, were integrated into a one‐week course, which provided students an opportunity to systematically understand the proteomic techniques and their applications in current scientific research. © 2018 by The International Union of Biochemistry and Molecular Biology, 46:354–360, 2018.