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Design of a software for calculating isoelectric point of a polypeptide according to their net charge using the graphical programming language LabVIEW
Author(s) -
Tovar Glomen
Publication year - 2017
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21088
Subject(s) - isoelectric point , software , computer science , isoelectric focusing , point (geometry) , net (polyhedron) , charge (physics) , programming language , analytical chemistry (journal) , chemistry , chromatography , mathematics , biochemistry , physics , enzyme , geometry , quantum mechanics
A software to calculate the net charge and to predict the isoelectric point (pI) of a polypeptide is developed in this work using the graphical programming language LabVIEW. Through this instrument the net charges of the ionizable residues of the polypeptide chains of the proteins are calculated at different pH values, tabulated, pI is predicted and an Excel (‐xls) type file is generated. In this work, the experimental values of the pIs (pI) of different proteins are compared with the values of the pIs (pI) calculated graphically, achieving a correlation coefficient ( R ) of 0.934746 which represents a good reliability for a p < 0.01. In this way the generated program can constitute an instrument applicable in the laboratory, facilitating the calculation to graduate students and junior researchers. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(1):39–46, 2018.