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A comprehensive experiment for molecular biology: Determination of single nucleotide polymorphism in human REV3 gene using PCR–RFLP
Author(s) -
Zhang Xu,
Shao Meng,
Gao Lu,
Zhao Yuanyuan,
Sun Zixuan,
Zhou Liping,
Yan Yongmin,
Shao Qixiang,
Xu Wenrong,
Qian Hui
Publication year - 2017
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.21037
Subject(s) - biology , restriction fragment length polymorphism , snp genotyping , single nucleotide polymorphism , genotyping , computational biology , snp , genetics , gene , microbiology and biotechnology , polymerase chain reaction , genotype
Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP. By combining these activities, the students are not only able to learn a series of biotechniques in molecular biology, but also acquire the ability to link the learned knowledge with practical applications. This comprehensive experiment will help the medical students improve the conceptual understanding of SNP and the technical understanding of SNP detection. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(4):299–304, 2017.