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Laboratory course on Streptomyces genetics and secondary metabolism
Author(s) -
Siitonen Vilja,
Räty Kaj,
MetsäKetelä Mikko
Publication year - 2016
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20970
Subject(s) - secondary metabolism , protoplast , streptomyces , biology , microbiology and biotechnology , biochemistry , streptomycetaceae , actinomycetales , bacteria , genetics , gene , biosynthesis
The “ Streptomyces genetics and secondary metabolism” laboratory course gives an introduction to the versatile soil dwelling Gram‐positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces ; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492–499, 2016.